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Cellosaurus LUMCi004-A-1 (CVCL_C6UC)

[Text version]
Cell line name LUMCi004-A-1
Synonyms LU99_AAVS1-bxb-v2
Accession CVCL_C6UC
Resource Identification Initiative To cite this cell line use: LUMCi004-A-1 (RRID:CVCL_C6UC)
Comments From: Leiden University Medical Center; Leiden; Netherlands.
Population: Caucasian.
Characteristics: Contains a platform to precisely integrate DNA payloads into the cell genome. Using CRISPR/Cas9 a STRAIGHT-IN v2 construct (loxP-EBFP2-attP(bxb1)-BleoR-lox257) was introduced in one of the alleles of the AAVS1 safe harbor locus (PubMed=36495703).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; DVMQ7; EBFP2 (Note=Blue fluorescent protein derived from GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UK77 (LUMCi004-A)
Sex of cell Female
Age at sampling 30-34Y
Category Induced pluripotent stem cell
Publications

PubMed=36495703; DOI=10.1016/j.scr.2022.102991
Blanch-Asensio A., van der Vaart B., Vinagre M., Groen E., Arendzen C.H., Freund C.M.A.H., Geijsen N., Mummery C.L., Davis R.P.
Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads.
Stem Cell Res. 66:102991-102991(2023)

Cross-references
Cell line databases/resources hPSCreg; LUMCi004-A-1
Biological sample resources BioSamples; SAMEA112164540
Encyclopedic resources Wikidata; Q117704634
Entry history
Entry creation21-Mar-2023
Last entry update10-Sep-2024
Version number3