ID   CCMB-Me-14-STGG-A1
AC   CVCL_C4SE
SY   STGG
DR   Wikidata; Q116048697
RX   PubMed=36063726;
CC   Characteristics: Using CRISPR/Cas9 a T2A-H2B-tdTomato construct was inserted at the C-terminus of one allele of Sox2 and a H2B-GFP-SV40-Bsd cassette was inserted at the C-terminus of one allele of Gata6 (PubMed=36063726).
CC   Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; 4761; H2BC11.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
CC   Breed/subspecies: 129P2/Ola.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_9108 ! ES-E14TG2a
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 15-12-22; Last updated: 10-09-24; Version: 4
//
RX   PubMed=36063726; DOI=10.1016/j.scr.2022.102900;
RA   Vishnu V.V., Shah H.S., Kumari S., Chandra Shekar P.;
RT   "CRISPR engineered mouse embryonic stem cells with Sox2-tdTomato and
RT   Gata6-GFP knock-in for endoderm differentiation.";
RL   Stem Cell Res. 64:102900-102900(2022).
//