ID   WAe009-A-92
AC   CVCL_C4RV
SY   Tet-on-ETV2 H9; NCCDFWe010-A-92
DR   BioSamples; SAMEA110358604
DR   hPSCreg; WAe009-A-92
DR   Wikidata; Q116050776
RX   PubMed=36463633;
CC   From: National Center for Cardiovascular Diseases and Fuwai Hospital; Beijing; China.
CC   Characteristics: Expresses ETV2 induced by doxycyclin. Transduced with a Tre-hETV2-mPGK-PuroR-rtTA-T2A-EF1a construct that contains the reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 (PubMed=36463633).
CC   Transfected with: HGNC; 3491; ETV2.
CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9773 ! WA09
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 15-12-22; Last updated: 29-06-23; Version: 3
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RX   PubMed=36463633; DOI=10.1016/j.scr.2022.102985;
RA   Zhou M., Ni J., Huang P.-F., Liu X.-J.;
RT   "Generation of a doxycycline-inducible ETV2 expression cell line using
RT   PiggyBac transposase system.";
RL   Stem Cell Res. 66:102985-102985(2023).
//