Cellosaurus cell line F1G (CVCL

Cellosaurus F1G (CVCL_C4MU)

[Text version]

Cell line name

F1G

Accession

CVCL_C4MU

Resource Identification Initiative

To cite this cell line use: F1G (RRID:CVCL_C4MU)

Comments

Group: Non-human primate cell line.
Group: SARS-CoV-2 research cell line.
Characteristics: Transduced with a fluorescent reporter (F1G) which is a construct that contains the substrate of the nsp5 protease (SAVLQSGF) between a NLS-eGFP and a membrane anchor. Thus GFP, upon cleavage of the nsp5 protease site by the SARS-CoV-2 main protease, re-localizes from the cytoplasm in non-infected cells to the nucleus in infected cells (PubMed=36246297).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Derived from site: In situ; Kidney, epithelium; UBERON=UBERON_0004819.
Cell type: Epithelial cell of kidney; CL=CL_0002518.

Species of origin

Chlorocebus sabaeus (Green monkey) (Simia sabaea) (NCBI Taxonomy: 60711)

Hierarchy

Parent: CVCL_0059 (Vero)
Children:

CVCL_C4MV (F1G-red)

Sex of cell

Female

Age at sampling

Adult