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Cellosaurus HeLa HFT/TO-PRKN PINK1-/- (CVCL_C4ES)

[Text version]
Cell line name HeLa HFT/TO-PRKN PINK1-/-
Accession CVCL_C4ES
Resource Identification Initiative To cite this cell line use: HeLa HFT/TO-PRKN PINK1-/- (RRID:CVCL_C4ES)
Comments Population: African American.
Knockout cell: Method=TALEN; HGNC; HGNC:14581; PINK1.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:8607; PRKN (Note=With 3xFLAG and HA tags).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C4EQ (HeLa HFT/TO-PRKN)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=25284222; DOI=10.1016/j.molcel.2014.09.007; PMCID=PMC4254048
Ordureau A., Sarraf S.A., Duda D.M., Heo J.-M., Jedrychowski M.P., Sviderskiy V.O., Olszewski J.L., Koerber J.T., Xie T., Beausoleil S.A., Wells J.A., Gygi S.P., Schulman B.A., Harper J.W.
Quantitative proteomics reveal a feedforward mechanism for mitochondrial PARKIN translocation and ubiquitin chain synthesis.
Mol. Cell 56:360-375(2014)

Cross-references
Encyclopedic resources Wikidata; Q116049308
Entry history
Entry creation15-Dec-2022
Last entry update19-Dec-2024
Version number6