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Cellosaurus MSHRIe002-A-SOX17 control 2 (CVCL_C3L7)

[Text version]
Cell line name MSHRIe002-A-SOX17 control 2
Synonyms CA2 NoCre Sox17E
Accession CVCL_C3L7
Resource Identification Initiative To cite this cell line use: MSHRIe002-A-SOX17 control 2 (RRID:CVCL_C3L7)
Comments Characteristics: Transfected with a loxP-BetaGeo(lacZ-neo)-polyA-loxP-SOX17-IRES-PuroR-polyA construct. This control cell line can be selected via neomycin resistance and does not overexpress SOX17 as this would require Cre-mediated excision of the loxP flanked betaGeo (PubMed=18682240).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 18122; SOX17.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B845 (MSHRIe002-A)
Children:
CVCL_C3L9 (MSHRIe002-A-SOX17 overexpressing 2)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=18682240; DOI=10.1016/j.stem.2008.06.018
Seguin C.A., Draper J.S., Nagy A., Rossant J.
Establishment of endoderm progenitors by SOX transcription factor expression in human embryonic stem cells.
Cell Stem Cell 3:182-195(2008)

Cross-references
Encyclopedic resources Wikidata; Q114312322
Gene expression databases GEO; GSM272921
Entry history
Entry creation22-Sep-2022
Last entry update10-Sep-2024
Version number5