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Cellosaurus MSHRIe001-A-SOX7 control 1 (CVCL_C3L2)

[Text version]
Cell line name MSHRIe001-A-SOX7 control 1
Synonyms CA1 NoCre Sox7H
Accession CVCL_C3L2
Resource Identification Initiative To cite this cell line use: MSHRIe001-A-SOX7 control 1 (RRID:CVCL_C3L2)
Comments Characteristics: Transfected with a loxP-BetaGeo(lacZ-neo)-polyA-loxP-SOX7-IRES-PuroR-polyA construct. This control cell line can be selected via neomycin resistance and does not overexpress SOX7 as this would require Cre-mediated excision of the loxP flanked betaGeo (PubMed=18682240).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:18196; SOX7.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B844 (MSHRIe001-A)
Children:
CVCL_C3L4 (MSHRIe001-A-SOX7 overexpressing 1)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=18682240; DOI=10.1016/j.stem.2008.06.018
Seguin C.A., Draper J.S., Nagy A., Rossant J.
Establishment of endoderm progenitors by SOX transcription factor expression in human embryonic stem cells.
Cell Stem Cell 3:182-195(2008)

Cross-references
Encyclopedic resources Wikidata; Q114312317
Gene expression databases GEO; GSM272915
Entry history
Entry creation22-Sep-2022
Last entry update19-Dec-2024
Version number6