Cellosaurus HCT-116 Safe Harbor Landing Pad (CVCL_C3EY)
Cell line name | HCT-116 Safe Harbor Landing Pad |
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Accession | CVCL_C3EY |
Resource Identification Initiative | To cite this cell line use: HCT-116 Safe Harbor Landing Pad (RRID:CVCL_C3EY) |
Comments | Population: Caucasian. Characteristics: Using ZFN a fluorescent mKATE2 tag flanked by Cre-lox sites (LoxN-EF1a-Lox2272-mKATE2-LoxP) was introduced in the AAVS1 safe harbor locus. The mKATE2 tag can be exchanged for a payload of the user's choice using Cre recombinase and a targeting vector with appropriate lox sites (Sigma-Aldrich=CLL1222). Doubling time: ~22 hours (Sigma-Aldrich; CLL1222). Genetic integration: Method=ZFN; Gene=FPbase; DBBO8; mKate2 (Note=Bright red fluorescent protein derived from eqFP578). Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
Sequence variations |
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Disease | Colon carcinoma (NCIt: C4910) |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_0291 (HCT 116) |
Sex of cell | Male |
Age at sampling | 48Y |
Category | Cancer cell line |
Cross-references | |
Cell line collections (Providers) | Sigma-Aldrich; CLL1222 |
Cell line databases/resources | cancercelllines; CVCL_C3EY |
Encyclopedic resources | Wikidata; Q114311572 |
Entry history | |
Entry creation | 22-Sep-2022 |
Last entry update | 19-Dec-2024 |
Version number | 7 |