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Cellosaurus A549 Safe Harbor Landing Pad (CVCL_C3EV)

[Text version]
Cell line name A549 Safe Harbor Landing Pad
Accession CVCL_C3EV
Resource Identification Initiative To cite this cell line use: A549 Safe Harbor Landing Pad (RRID:CVCL_C3EV)
Comments Population: Caucasian.
Characteristics: Using ZFN a fluorescent mKATE2 tag flanked by Cre-lox sites (LoxN-EF1a-Lox2272-mKATE2-LoxP) was introduced in the AAVS1 safe harbor locus. The mKATE2 tag can be exchanged for a payload of the user's choice using Cre recombinase and a targeting vector with appropriate lox sites (Sigma-Aldrich=CLL1220).
Doubling time: ~22 hours (Sigma-Aldrich; CLL1220).
Genetic integration: Method=ZFN; Gene=FPbase; DBBO8; mKate2 (Note=Bright red fluorescent protein derived from eqFP578).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Sigma-Aldrich; CLL1220
Cell line databases/resources cancercelllines; CVCL_C3EV
Encyclopedic resources Wikidata; Q114310530
Entry history
Entry creation22-Sep-2022
Last entry update19-Dec-2024
Version number7