Cellosaurus U2OS BFP-LMNB1 GFP-TUBA1B RFP-ACTB (CVCL_C3C9)
| Cell line name | U2OS BFP-LMNB1 GFP-TUBA1B RFP-ACTB |
|---|---|
| Synonyms | U2OS BFP-LMNB1/GFP-TUBA1B/RFP-ACTB; U2OS LMNB1-TUBA1B-ACTB |
| Accession | CVCL_C3C9 |
| Resource Identification Initiative | To cite this cell line use: U2OS BFP-LMNB1 GFP-TUBA1B RFP-ACTB (RRID:CVCL_C3C9) |
| Comments | Population: Caucasian. Characteristics: Edited by ZFN at the LMNB1 locus to insert a TagBFP construct at the N-terminus of the gene, at the TUB1A1B locus to insert a TagGFP2 construct at the N-terminus of the gene and at the ACTB locus to insert a TagRFP construct at the N-terminus of the gene (Sigma-Aldrich=CLL1218). Genetic integration: Method=ZFN; Gene=FPbase; GJETS; TagGFP2 (Note=Green fluorescent protein derived from Aequorea macrodactyla GFP). Genetic integration: Method=ZFN; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578). Genetic integration: Method=ZFN; Gene=FPbase; S4HC8; TagRFP (Note=Red fluorescent protein derived from eqFP578). Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979. |
| Sequence variations |
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| Disease | Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_C3CB (U2OS BFP-LMNB1 RFP-ACTB) |
| Sex of cell | Female |
| Age at sampling | 15Y |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Sigma-Aldrich; CLL1218 |
| Encyclopedic resources | Wikidata; Q114313146 |
| Entry history | |
| Entry creation | 22-Sep-2022 |
| Last entry update | 10-Sep-2024 |
| Version number | 6 |