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Cellosaurus STBCi322-A-1 (CVCL_C1XW)

[Text version]
Cell line name STBCi322-A-1
Synonyms NKX6.1-GFP reporter line; NKX6.1-GFP clone 1a-10-3; AZi323-A-1
Accession CVCL_C1XW
Resource Identification Initiative To cite this cell line use: STBCi322-A-1 (RRID:CVCL_C1XW)
Comments From: AstraZeneca; Cambridge; United Kingdom.
Characteristics: Using CRISPR/Cas9 a T2A-GFP-LoxP-PGKp-Neo-LoxP construct was introduced at the C-terminus of NKX6-1. Subsequently the LoxP-PGKp-Neo-LoxP sequence was removed by Cre-mediated excision (PubMed=29734117).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Skin; UBERON=UBERON_0002097.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_ZX55 (STBCi322-A)
Sex of cell Female
Age at sampling 36Y
Category Induced pluripotent stem cell
Publications

PubMed=29734117; DOI=10.1016/j.scr.2018.04.010
Gupta S.K., Wesolowska-Andersen A., Ringgaard A.K., Jaiswal H., Song L.-Y., Hastoy B., Ingvorsen C., Taheri-Ghahfarokhi A., Magnusson B., Maresca M., Jensen R.R., Beer N.L., Fels J.J., Grunnet L.G., Thomas M.K., Gloyn A.L., Hicks R., McCarthy M.I., Hansson M., Honore C.
NKX6.1 induced pluripotent stem cell reporter lines for isolation and analysis of functionally relevant neuronal and pancreas populations.
Stem Cell Res. 29:220-231(2018)

Cross-references
Cell line databases/resources hPSCreg; STBCi322-A-1
Encyclopedic resources Wikidata; Q114313049
Entry history
Entry creation22-Sep-2022
Last entry update10-Sep-2024
Version number5