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Cellosaurus HEK 293T-Spike-HIV-TV (CVCL_C1D1)

[Text version]
Cell line name HEK 293T-Spike-HIV-TV
Accession CVCL_C1D1
Resource Identification Initiative To cite this cell line use: HEK 293T-Spike-HIV-TV (RRID:CVCL_C1D1)
Comments Characteristics: Transduced with a pT-PB-SARS-CoV-2-Spike-IRES-Bsd construct and a HIV-TV plasmid pUCHR-inGLuc-pgkhygro that encodes the intron-regulated HIV-based Gaussia luciferase.
Genetic integration: Method=Transduction; Gene=UniProtKB; P0DTC2; Human SARS coronavirus 2 (SARS-CoV-2) spike protein (S).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=35757815; DOI=10.1016/j.crmeth.2022.100252; PMCID=PMC9213030
Ssenyange G., Kerfoot M., Zhao M., Farhadian S., Chen S.-D., Peng L., Ren P., Dela Cruz C.S., Gupta S., Sutton R.E.
Development of an efficient reproducible cell-cell transmission assay for rapid quantification of SARS-CoV-2 spike interaction with hACE2.
Cell Rep. Methods 2:100252.1-100252.19(2022)

Cross-references
Encyclopedic resources Wikidata; Q114311578
Entry history
Entry creation22-Sep-2022
Last entry update10-Sep-2024
Version number6