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Cellosaurus HEK293T Tet-off FLTau (CVCL_C1CR)

[Text version]
Cell line name HEK293T Tet-off FLTau
Synonyms Tet-FLTau
Accession CVCL_C1CR
Resource Identification Initiative To cite this cell line use: HEK293T Tet-off FLTau (RRID:CVCL_C1CR)
Comments Characteristics: Expresses Tau/MAPT under the control of a Tet-regulated promoter (PubMed=36732333).
Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:6893; MAPT (Note=With p.Pro301Leu and p.Val337Met).
Genetic integration: Method=Transfection; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=36732333; DOI=10.1038/s41467-023-36058-2; PMCID=PMC9894937
Saha I., Yuste-Checa P., Da Silva Padilha M., Guo Q., Korner R., Holthusen H., Trinkaus V.A., Dudanova I., Fernandez-Busnadiego R., Baumeister W.P., Sanders D.W., Gautam S., Diamond M.I., Hartl F.-U., Hipp M.S.
The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system.
Nat. Commun. 14:560.1-560.17(2023)

Cross-references
Encyclopedic resources Wikidata; Q114311580
Entry history
Entry creation22-Sep-2022
Last entry update19-Dec-2024
Version number8