Cellosaurus BIONi010-C-NKX2.5-T2A-Nluc-73.59 (CVCL_C0VU)
| Cell line name | BIONi010-C-NKX2.5-T2A-Nluc-73.59 |
|---|---|
| Synonyms | #73.59 |
| Accession | CVCL_C0VU |
| Resource Identification Initiative | To cite this cell line use: BIONi010-C-NKX2.5-T2A-Nluc-73.59 (RRID:CVCL_C0VU) |
| Comments | Population: African. Biotechnology: Part of the PluriLum assay which is used for predicting developmental toxicity which relies on the formation of embryoid bodies (EBs) and the differentiation of these into cardiomyocytes (PubMed=33660062). Characteristics: Using CRISPR/Cas9 a T2A-Nluc construct was inserted at the C-terminus of both alleles of NKX2-5 (PubMed=33660062). Genetic integration: Method=CRISPR/Cas9; Gene=NanoLuc (Note=19 kDa ATP-independent bright luminescent protein derived from O.gracilirostris). Derived from site: In situ; Skin, dermis; UBERON=UBERON_0002067. Cell type: Fibroblast of skin; CL=CL_0002620. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_1E68 (BIONi010-C) |
| Sex of cell | Male |
| Age at sampling | 18Y |
| Category | Induced pluripotent stem cell |
| Publications | PubMed=33660062; DOI=10.1007/s00204-021-03018-y; PMCID=PMC8113199 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q114310753 |
| Entry history | |
| Entry creation | 22-Sep-2022 |
| Last entry update | 10-Sep-2024 |
| Version number | 5 |