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Cellosaurus HT22 TR/TO-rKlf9 clone 2.1 (CVCL_C0UA)

[Text version]
Cell line name HT22 TR/TO-rKlf9 clone 2.1
Synonyms HT22 [TR/TO-rKlf9] clone 2.1; HT-22 [TR/TO-rKlf9] clone 2.1; HT22 [TR/TO-Klf9] line 2-1; HT22[TR/TO-Klf9]
Accession CVCL_C0UA
Resource Identification Initiative To cite this cell line use: HT22 TR/TO-rKlf9 clone 2.1 (RRID:CVCL_C0UA)
Comments Characteristics: Doxycycline induced expression of Klf9. Transfected with both pCDNA4:TO-Klf9 containing CMV-tetO2-Klf9 and the zeocin resistance gene (ble) and pCDNA6:TR containing tetR and the blasticidin resistance gene Bsr.
Genetic integration: Method=Transfection; Gene=RGD; 70934; Klf9.
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Derived from site: In situ; Brain, hippocampus; UBERON=UBERON_0002421.
Cell type: Neuron; CL=CL_0000540.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_0321 (HT22)
Category Transformed cell line
Web pages https://umich.flintbox.com/technologies/b40d696c-0810-48f8-84f4-3c312dda7e8b
Publications

PubMed=28407733; DOI=10.1186/s12864-017-3640-7; PMCID=PMC5390390
Knoedler J.R., Subramani A., Denver R.J.
The Kruppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding.
BMC Genomics 18:299.1-299.22(2017)

PubMed=32241200; DOI=10.1177/0748730420913205; PMCID=PMC9199006
Knoedler J.R., Avila-Mendoza J., Subramani A., Denver R.J.
The paralogous Kruppel-like factors 9 and 13 regulate the mammalian cellular circadian clock output gene Dbp.
J. Biol. Rhythms 35:257-274(2020)

Cross-references
Cell line collections (Providers) Kerafast; EMU045
Encyclopedic resources Wikidata; Q112929760
Entry history
Entry creation23-Jun-2022
Last entry update19-Dec-2024
Version number7