Cellosaurus ACTOne cAMP-PDE4C (CVCL_C0U4)
| Cell line name | ACTOne cAMP-PDE4C |
|---|---|
| Synonyms | ACTOne PDE4C; PDE4C ACTOne; CHO-K1-CNG-PDE4C |
| Accession | CVCL_C0U4 |
| Resource Identification Initiative | To cite this cell line use: ACTOne cAMP-PDE4C (RRID:CVCL_C0U4) |
| Comments | Characteristics: Expresses a modified cyclic nucleotide gated (CNG) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization. Allows sensitive detection of signaling by Gs, Gi or Gq-coupled receptors (Codex BioSolutions). Characteristics: Expresses a constitutively active over-expressed Gs-coupled GPCR. Genetic integration: Method=Transfection/transduction; Gene=RGD; 2367; Cnga2 (Note=With p.Phe68Ala, p.Trp81Ala, p.Cys460Trp and p.Glu583Met = oCNG-NC). Genetic integration: Method=Transfection/transduction; Gene=HGNC; 8782; PDE4C. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Discontinued: Codex Biosolutions; Catalog number CL-02-PDE4C. Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064. |
| Species of origin | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029) |
| Hierarchy | Parent: CVCL_C0RV (CHO-K1-CNG-Gs) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q112929140 |
| Entry history | |
| Entry creation | 23-Jun-2022 |
| Last entry update | 10-Sep-2024 |
| Version number | 6 |