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Cellosaurus ACTOne cAMP-PDE4B (CVCL_C0U3)

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Cell line name ACTOne cAMP-PDE4B
Synonyms ACTOne PDE4B; PDE4B ACTOne; CHO-K1-CNG-PDE4B
Accession CVCL_C0U3
Resource Identification Initiative To cite this cell line use: ACTOne cAMP-PDE4B (RRID:CVCL_C0U3)
Comments Characteristics: Expresses a modified cyclic nucleotide gated (CNG) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization. Allows sensitive detection of signaling by Gs, Gi or Gq-coupled receptors (Codex BioSolutions).
Characteristics: Expresses a constitutively active over-expressed Gs-coupled GPCR.
Genetic integration: Method=Transfection/transduction; Gene=RGD; 2367; Cnga2 (Note=With p.Phe68Ala, p.Trp81Ala, p.Cys460Trp and p.Glu583Met = oCNG-NC).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:8781; PDE4B.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_C0RV (CHO-K1-CNG-Gs)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages http://www.codexbiosolutions.com/wp-content/uploads/cb-81200-106.pdf
Cross-references
Encyclopedic resources Wikidata; Q112929139
Entry history
Entry creation23-Jun-2022
Last entry update19-Dec-2024
Version number7