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Cellosaurus 3T3J2NHP (CVCL_C0R6)

[Text version]
Cell line name 3T3J2NHP
Accession CVCL_C0R6
Resource Identification Initiative To cite this cell line use: 3T3J2NHP (RRID:CVCL_C0R6)
Comments Characteristics: Used a feeder cells to support transduced keratinocyte populations during selection with one of G418, hygromycin or puromycin.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: Swiss albino.
Hierarchy Parent: CVCL_W667 (3T3-J2)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
Publications

PubMed=10648628; DOI=10.1128/mcb.20.4.1436-1447.2000; PMCID=PMC85304
Dickson M.A., Hahn W.C., Ino Y., Ronfard V., Wu J.Y., Weinberg R.A., Louis D.N., Li F.P., Rheinwald J.G.
Human keratinocytes that express hTERT and also bypass a p16(INK4a)-enforced mechanism that limits life span become immortal yet retain normal growth and differentiation characteristics.
Mol. Cell. Biol. 20:1436-1447(2000)

Cross-references
Encyclopedic resources Wikidata; Q112926974
Entry history
Entry creation23-Jun-2022
Last entry update10-Sep-2024
Version number6