ID   PC-DR-LUC
AC   CVCL_C0Q0
DR   Wikidata; Q112930254
RX   PubMed=17532181;
CC   Biotechnology: Used in an assay that can be used to identify compounds that have thyromimetic or TH-antagonistic effects, and that could be useful for detecting thyroid-disrupting chemicals in environmental samples (PubMed=17532181).
CC   Characteristics: Expression of luciferase is under the control of a promoter containing two thyroid hormone response elements (TREs) separated by four nucleotides (DR4) followed by a SV40 promoter (PubMed=17532181).
CC   Genetic integration: Method=Transduction; Gene=CGNC; 155; THRA.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
CC   Derived from site: In situ; Adrenal gland; UBERON=UBERON_0002369.
CC   Breed/subspecies: NEDH; RS=RS_0000603.
DI   NCIt; C121568; Rat adrenal gland pheochromocytoma
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_C0PZ ! PC12 cA4
SX   Male
AG   Age unspecified
CA   Cancer cell line
DT   Created: 23-06-22; Last updated: 14-08-25; Version: 10
//
RX   PubMed=17532181; DOI=10.1016/j.tiv.2007.03.020;
RA   Jugan, Mary-Line
RA   Levy-Bimbot, Maya
RA   Pomerance, Martine
RA   Tamisier-Karolak, Sara
RA   Blondeau, Jean-Paul
RA   Levi, Yves
RT   "A new bioluminescent cellular assay to measure the transcriptional
RT   effects of chemicals that modulate the alpha-1 thyroid hormone
RT   receptor.";
RL   Toxicol. In Vitro 21:1197-1205(2007).
//