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Cellosaurus BIHi001-A-2 (CVCL_C0NJ)

[Text version]
Cell line name BIHi001-A-2
Synonyms AAVS1-Puro-CAG-eGFP targeted hiPSC
Accession CVCL_C0NJ
Resource Identification Initiative To cite this cell line use: BIHi001-A-2 (RRID:CVCL_C0NJ)
Comments From: Berlin Institute of Health; Berlin; Germany.
Population: Caucasian.
Characteristics: Using CRISPR/Cas9 a PuroR-CAG-eGFP construct was introduced in the AAVS1 safe harbor locus (PubMed=32384610).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_IT54 (BIHi001-A)
Sex of cell Male
Age at sampling <1M
Category Induced pluripotent stem cell
Publications

PubMed=32384610; DOI=10.3390/genes11050511; PMCID=PMC7288465
Surun D., Schneider A., Mircetic J., Neumann K., Lansing F., Paszkowski-Rogacz M., Hanchen V., Lee-Kirsch M.A., Buchholz F.
Efficient generation and correction of mutations in human iPS cells utilizing mRNAs of CRISPR base editors and prime editors.
Genes (Basel) 11:511.1-511.19(2020)

Cross-references
Cell line databases/resources hPSCreg; BIHi001-A-2
Encyclopedic resources Wikidata; Q112929326
Entry history
Entry creation23-Jun-2022
Last entry update10-Sep-2024
Version number5