Cellosaurus SCVIi038-A (CVCL_C0J0)
| Cell line name | SCVIi038-A |
|---|---|
| Synonyms | AICS-0052-003-iKRABdCas9; ipsc WTC-my17 KRAB-mcherry clone 1 |
| Accession | CVCL_C0J0 |
| Resource Identification Initiative | To cite this cell line use: SCVIi038-A (RRID:CVCL_C0J0) |
| Comments | From: Stanford Cardiovascular Institute; Palo Alto; USA. Population: Japanese. Characteristics: Using CRISPR/Cas9 a doxycycline-inducible dCas9-KRAB-mCherry cassette was introduced in both alleles of the AAVS1 safe harbor locus (PubMed=35325819). Characteristics: Using CRISPR/Cas9 MYL7 has been endogenously tagged in one allele at the C-terminus with mEGFP. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511. Cell type: Fibroblast of skin; CL=CL_0002620. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_UD15 (WTC-mEGFP-MYL7-cl3) |
| Sex of cell | Male |
| Age at sampling | 30Y |
| Category | Induced pluripotent stem cell |
| Publications | PubMed=35325819; DOI=10.1016/j.scr.2022.102754; PMCID=PMC9169598 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; SCVIi038-A |
| Encyclopedic resources | Wikidata; Q112930358 |
| Entry history | |
| Entry creation | 23-Jun-2022 |
| Last entry update | 10-Sep-2024 |
| Version number | 6 |