ID   MRIi003-A-5
AC   CVCL_C0IY
SY   AAVS-CAG-VSFP heterozygous; AAVS1-CAG-VSFP-hetero
DR   BioSamples; SAMEA10056289
DR   hPSCreg; MRIi003-A-5
DR   Wikidata; Q112930126
RX   PubMed=35421847;
CC   From: Klinikum rechts der Isar, Technical University of Munich; Munich; Germany.
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9 a CAG-promoter driven voltage-sensing fluorescent protein (VSFP) that consist of of a voltage-sensing transmembrane domain fused to a pair of fluorescent proteins: Clover and mRuby2 was integrated into one allele of the AAVS1 safe harbor locus (PubMed=35421847).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 4Z641; Clover (Note=Basic green/yellow fluorescent protein derived from GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 8MJ78; mRuby2 (Note=Bright red fluorescent protein derived from eqFP61).
CC   Misspelling: MRli003-A-5; PubMed=35421847.
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A8MN ! MRIi003-A
SX   Male
AG   Age unspecified
CA   Induced pluripotent stem cell
DT   Created: 23-06-22; Last updated: 10-09-24; Version: 7
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RX   PubMed=35421847; DOI=10.1016/j.scr.2022.102785;
RA   Zhang F.-F., Meier A.B., Sinnecker D., Engelhardt S., Lipp P.,
RA   Laugwitz K.-L., Dorn T., Moretti A.;
RT   "Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6)
RT   voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of
RT   the AAVS1 locus.";
RL   Stem Cell Res. 61:102785-102785(2022).
//