ID   293EL-APP*
AC   CVCL_C0I9
DR   Wikidata; Q112926925
RX   PubMed=36245040;
CC   Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:620; APP.
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:620; APP (Note=With p.Lys670Asn, p.Met671Leu, p.Thr719Asn = APPSw/T700N).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:3185; EEA1 (Note=With a N-terminal FLAG tag).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_C0I8 ! 293EL-APP-/-
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 23-06-22; Last updated: 19-12-24; Version: 7
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RX   PubMed=36245040; DOI=10.1038/s41467-022-33881-x; PMCID=PMC9573879;
RA   Park H., Hundley F.V., Yu Q., Overmyer K.A., Brademan D.R.,
RA   Serrano L., Paulo J.A., Paoli J.C., Swarup S., Coon J.J., Gygi S.P.,
RA   Harper J.W.;
RT   "Spatial snapshots of amyloid precursor protein intramembrane
RT   processing via early endosome proteomics.";
RL   Nat. Commun. 13:6112.1-6112.21(2022).
//