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Cellosaurus NSI002-B (CVCL_C0HP)

[Text version]
Cell line name NSI002-B
Synonyms AAVS1-EGFP-Di
Accession CVCL_C0HP
Resource Identification Initiative To cite this cell line use: NSI002-B (RRID:CVCL_C0HP)
Comments From: Stem Cell and Neurobiology Lab, National Centre for Cell Science; Pune; India.
Population: Caucasian.
Characteristics: Using ZFN a PuroR-CAG-EGFP cassette was introduced in the AAVS1 safe harbor locus (PubMed=35961104).
Karyotypic information: Lost the third copy of chromosome 21 (from parent cell line).
Genetic integration: Method=ZFN; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=ZFN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Chest, skin; UBERON=UBERON_0001868.
Cell type: Fibroblast of skin; CL=CL_0002620.
Disease Down syndrome (NCIt: C2993)
Down syndrome (ORDO: Orphanet_870)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C1XZ (DS1-iPS4-Disomic)
Sex of cell Male
Age at sampling 1Y
Category Induced pluripotent stem cell
Publications

PubMed=35961104; DOI=10.1016/j.scr.2022.102890
Sharma V., Nehra S., Singhal N.
Generation of AAVS1-EGFP reporter cell lines from an isogenic pair of trisomy 21 and euploid human iPSCs.
Stem Cell Res. 64:102890-102890(2022)

Cross-references
Cell line databases/resources hPSCreg; NSI002-B
Encyclopedic resources Wikidata; Q112930237
Entry history
Entry creation23-Jun-2022
Last entry update10-Sep-2024
Version number6