Cellosaurus SCVIi028-A-1 (CVCL_C0EX)
| Cell line name | SCVIi028-A-1 |
|---|---|
| Synonyms | SCVi-15S-PE2 |
| Accession | CVCL_C0EX |
| Resource Identification Initiative | To cite this cell line use: SCVIi028-A-1 (RRID:CVCL_C0EX) |
| Comments | From: Stanford Cardiovascular Institute; Palo Alto; USA. Characteristics: Dox-inducible expression of the prime editor protein (PE2) which is a fusion between the S.pyogenes Cas9(H840A) and an engineered Moloney murine leukemia virus (MMLV) reverse transcriptase domain. PE2 enables gene editing at potentially any locus in the genome by transfecting a pegRNA specific for the desired target locus (PubMed=34875545). Characteristics: Using CRISPR/Cas9 a T2A-Neo-CAG-rtTA-BFP-P2A-PE2-TRE3G construct has been inserted at the AAVS1 safe harbor locus (PubMed=34875545). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.His840Ala). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; SDS4K; BFP (Note=Blue fluorescent protein derived from GFP). Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_C0EW (SCVIi028-A) |
| Sex of cell | Male |
| Age at sampling | 21Y |
| Category | Induced pluripotent stem cell |
| Publications | PubMed=34875545; DOI=10.1016/j.scr.2021.102610; PMCID=PMC9126997 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; SCVIi028-A-1 |
| Encyclopedic resources | Wikidata; Q112930355 |
| Entry history | |
| Entry creation | 23-Jun-2022 |
| Last entry update | 10-Sep-2024 |
| Version number | 7 |