Cellosaurus ESIBIe003-A-10 (CVCL_C0CN)
| Cell line name | ESIBIe003-A-10 |
|---|---|
| Synonyms | HES3 INS-GFP GCG-mCh; INSGFP/wGCGmCh/w hESC; MCRIe004-A-10 |
| Accession | CVCL_C0CN |
| Resource Identification Initiative | To cite this cell line use: ESIBIe003-A-10 (RRID:CVCL_C0CN) |
| Comments | From: Murdoch Children's Research Institute; Melbourne; Australia. Characteristics: Reporter cell line that expresses GFP and mCherry fluorescent proteins under the control of the endogenous insulin (INS) and glucagon (GCG) loci respectively. Using homologous recombination an EGFP reporter gene was introduced in one allele of the INS locus. Using TALEN a mCherry reporter was introduced at the N-terminus in one allele of the GCG locus (PubMed=34619644). Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Omics: SNP array analysis. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_XA15 (HES-3 INSGFP/w) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=34619644; DOI=10.1016/j.scr.2021.102547 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; ESIBIe003-A-10 |
| Biological sample resources | BioSamples; SAMEA9462434 |
| Encyclopedic resources | Wikidata; Q112929540 |
| Entry history | |
| Entry creation | 23-Jun-2022 |
| Last entry update | 10-Sep-2024 |
| Version number | 5 |