Cellosaurus logo
expasy logo

Cellosaurus WAe001-A-77 (CVCL_C0CB)

[Text version]
Cell line name WAe001-A-77
Synonyms H1 RYBP-FLAG-HA KI; GIBHe007-A-77
Accession CVCL_C0CB
Resource Identification Initiative To cite this cell line use: WAe001-A-77 (RRID:CVCL_C0CB)
Comments From: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences; Guangzhou; China.
Characteristics: Using CRISPR/Cas9 a FLAG-HA tag followed by a PGKp-PuroR cassette were introduced at the C-terminus of both alleles of RYBP (PubMed=35537243).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=35537243; DOI=10.1016/j.scr.2022.102803
Zhang J.-Y., Zhou T.-C., Shan Y.-L., Pan G.-J.
Generation of RYBP FLAG-HA knock-in human embryonic stem cell line through CRISPR/Cas9-mediated homologous recombination.
Stem Cell Res. 62:102803-102803(2022)

Cross-references
Cell line databases/resources hPSCreg; WAe001-A-77
Biological sample resources BioSamples; SAMEA12946815
Encyclopedic resources Wikidata; Q112930548
Entry history
Entry creation23-Jun-2022
Last entry update10-Sep-2024
Version number6