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Cellosaurus ES-R1 CAG-S-hPPARgamma2 (CVCL_BW93)

[Text version]
Cell line name ES-R1 CAG-S-hPPARgamma2
Accession CVCL_BW93
Resource Identification Initiative To cite this cell line use: ES-R1 CAG-S-hPPARgamma2 (RRID:CVCL_BW93)
Comments Characteristics: The transfected construct consist of a ubiquitous CAGGS (hybrid chicken beta actin and cytomegalovirus) promoter, a loxP-STOP-loxP (LSL) cassette and the Myc-FLAG-tagged NR/CoR cDNA followed by a polyadenylation signal. The contruct is silent and the transfected gene can be conditionally overexpressed after cre recombinase mediated activation.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:9236; PPARG.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: 129X1/SvJ x 129S1/Sv-Oca2+Tyr+KitlSl-J.
Hierarchy Parent: CVCL_2167 (ES-R1)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=20382891; DOI=10.1210/me.2010-0068; PMCID=PMC2875802
Wu S.-P., Lee D.-K., DeMayo F.J., Tsai S.Y., Tsai M.-J.
Generation of ES cells for conditional expression of nuclear receptors and coregulators in vivo.
Mol. Endocrinol. 24:1297-1304(2010)

Cross-references
Encyclopedic resources Wikidata; Q54832458
Entry history
Entry creation13-Jul-2016
Last entry update19-Dec-2024
Version number10