ID   SRD-15/pInsig-2
AC   CVCL_B7PF
DR   Wikidata; Q112930422
RX   PubMed=15866869;
CC   Characteristics: Transfected with INSIG2 followed by 6 tandem copies of the Myc epitope (PubMed=15866869).
CC   Selected for resistance to: ChEBI; CHEBI:77317; 4-[2,2-bis(diethoxyphosphoryl)ethenyl]-2,6-ditert-butylphenol] (SR12813).
CC   Selected for resistance to: ChEBI; CHEBI:42977; 25-hydroxycholesterol.
CC   Sequence variation: Gene deletion; UniProtKB; Q8CFA6; Insig1; Zygosity=Heterozygous; Note=The 2nd allele has a loss of function (from parent cell line).
CC   Sequence variation: Gene deletion; UniProtKB; Q8CFA5; Insig2; Zygosity=Heterozygous (PubMed=15866869).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; 20452; INSIG2.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCIt; C129643; Cesium-137 gamma radiation.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_B7PD ! SRD-15
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 23-06-22; Last updated: 10-09-24; Version: 7
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RX   PubMed=15866869; DOI=10.1074/jbc.M502989200;
RA   Lee P.C.W., Sever N., DeBose-Boyd R.A.;
RT   "Isolation of sterol-resistant Chinese hamster ovary cells with
RT   genetic deficiencies in both Insig-1 and Insig-2.";
RL   J. Biol. Chem. 280:25242-25249(2005).
//