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Cellosaurus SRD-14/pInsig-2 (CVCL_B7PC)

[Text version]
Cell line name SRD-14/pInsig-2
Accession CVCL_B7PC
Resource Identification Initiative To cite this cell line use: SRD-14/pInsig-2 (RRID:CVCL_B7PC)
Comments Characteristics: Transfected with INSIG2 followed by 6 tandem copies of the Myc epitope.
Selected for resistance to: ChEBI; CHEBI:77317; 4-[2,2-bis(diethoxyphosphoryl)ethenyl]-2,6-ditert-butylphenol] (SR12813).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 20452; INSIG2.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCIt; C129643; Cesium-137 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q8CFA6; Insig1; Zygosity=Heterozygous; Note=The 2nd allele has a loss of function (PubMed=15247248).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_B7PA (SRD-14)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=15247248; DOI=10.1074/jbc.M406406200
Sever N., Lee P.C.W., Song B.-L., Rawson R.B., DeBose-Boyd R.A.
Isolation of mutant cells lacking Insig-1 through selection with SR-12813, an agent that stimulates degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
J. Biol. Chem. 279:43136-43147(2004)

Cross-references
Encyclopedic resources Wikidata; Q112930419
Entry history
Entry creation23-Jun-2022
Last entry update10-Sep-2024
Version number6