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Cellosaurus 1E2 [CRISPR/Cas9 edited mouse hybridoma against influenza D virus HE] (CVCL_B6TR)

[Text version]
Cell line name 1E2 [CRISPR/Cas9 edited mouse hybridoma against influenza D virus HE]
Accession CVCL_B6TR
Resource Identification Initiative To cite this cell line use: 1E2 [CRISPR/Cas9 edited mouse hybridoma against influenza D virus HE] (RRID:CVCL_B6TR)
Comments Characteristics: Using CRISPR/Cas9 a bovine herpesvirus type 1 glycoprotein E (BoHV1gE)-GFP fusion was inserted in the C region of the Ig light chain V12.41/J4/C (PubMed=34171554).
Monoclonal antibody isotype: Not specified.
Monoclonal antibody target: UniProtKB; A0A140AT86; Influenza D virus (D/swine/Italy/199724-3/2015) hemagglutinin-esterase-fusion protein (HE).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q08101; Bovine herpesvirus 1 envelope glycoprotein E (gE).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_B6TP (1E1 [Mouse hybridoma against influenza D virus HE])
Category Hybridoma
Publications

PubMed=34171554; DOI=10.1016/j.vetimm.2021.110286
Schibeci Natoli Scialli N., Colitti B., Bertolotti L., Pezzoni G., Martignani E., Melega M., Brocchi E., Rosati S.
Genome editing of a hybridoma cell line via the CRISPR/Cas9 system: a new approach for constitutive high-level expression of heterologous proteins in eukaryotic system.
Vet. Immunol. Immunopathol. 238:110286.1-110286.8(2021)

Cross-references
Encyclopedic resources Wikidata; Q111732636
Entry history
Entry creation17-Mar-2022
Last entry update10-Sep-2024
Version number5