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Cellosaurus MHHi001-A-8 (CVCL_B6RK)

[Text version]
Cell line name MHHi001-A-8
Synonyms hiPSCGCaMP6f/Neo
Accession CVCL_B6RK
Resource Identification Initiative To cite this cell line use: MHHi001-A-8 (RRID:CVCL_B6RK)
Comments From: Hannover Medical School, LEBAO (MHH); Hannover; Germany.
Population: Caucasian.
Characteristics: Using TALEN a CAG-promoter driven GCamP6f and a pMYH6-Neo-pPGKp-HygroR construct were introduced in both alleles of the AAVS1 safe harbor locus (PubMed=35152180).
Genetic integration: Method=TALEN; Gene=FPbase; F5GKU; GCaMP6f (Note=Green fluorescent calcium sensor).
Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_QX51 (MHHi001-A)
Sex of cell Female
Age at sampling <1D
Category Induced pluripotent stem cell
Publications

PubMed=35152180; DOI=10.1016/j.scr.2022.102697
Ricci Signorini M.E., Szepes M., Melchert A., Bakar M., Merkert S., Haase A., Gohring G., Martin U., Gruh I.
Generation of human induced pluripotent stem cell lines encoding for genetically encoded calcium indicators RCaMP1h and GCaMP6f.
Stem Cell Res. 60:102697-102697(2022)

Cross-references
Cell line databases/resources hPSCreg; MHHi001-A-8
Encyclopedic resources Wikidata; Q112129316
Entry history
Entry creation17-Mar-2022
Last entry update10-Sep-2024
Version number6