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Cellosaurus KUIMSi012-A-2 (CVCL_B6RG)

[Text version]
Cell line name KUIMSi012-A-2
Synonyms GAPDH-tdT2
Accession CVCL_B6RG
Resource Identification Initiative To cite this cell line use: KUIMSi012-A-2 (RRID:CVCL_B6RG)
Comments From: Kyoto University; Kyoto; Japan.
Population: African American.
Characteristics: Using CRISPR/Cas9 a 2A-tdTomato construct was integrated at the C-terminus of both alleles of GAPDH (PubMed=35176664).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_LJ39 (1231A3)
Sex of cell Female
Age at sampling 29Y
Category Induced pluripotent stem cell
STR profile Source(s): PubMed=35176664

Markers:
AmelogeninX
CSF1PO10,13
D3S135814,15
D5S81813,14
D7S8209,10
D8S117913,17
D13S31711,12
D16S53911,12
D18S5115,17
D21S1131
FGA24,25
Penta D2.2,13
Penta E12,14
TH017,9
TPOX8,9
vWA15,16

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Publications

PubMed=35176664; DOI=10.1016/j.scr.2022.102704
Kamiya D., Yamashita T., Akaboshi T., Yamaguchi Y., Toyooka Y., Ikeya M.
Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions.
Stem Cell Res. 60:102704-102704(2022)

Cross-references
Cell line databases/resources hPSCreg; KUIMSi012-A-2
Encyclopedic resources Wikidata; Q111733567
Entry history
Entry creation17-Mar-2022
Last entry update10-Sep-2024
Version number6