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Cellosaurus iKLF1.2 (CVCL_B6RB)

[Text version]
Cell line name iKLF1.2
Accession CVCL_B6RB
Resource Identification Initiative To cite this cell line use: iKLF1.2 (RRID:CVCL_B6RB)
Comments Characteristics: Using ZFN a CAG-HA-KLF1-ERT2 construct has been inserted at the AAVS1 safe harbor locus so as to allow temporally controlled activation during macrophage differentiation.
Genetic integration: Method=ZFN; Gene=HGNC; 3467; ESR1 (Note=Ligand-binding domain with p.Gly400Val, p.Met543Ala and p.Leu544Ala = ET[T2]).
Genetic integration: Method=ZFN; Gene=HGNC; 6345; KLF1.
Genetic integration: Method=ZFN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UL47 (SFCi55)
Sex of cell Female
Category Induced pluripotent stem cell
Publications

PubMed=28026072; DOI=10.1002/stem.2562; PMCID=PMC5396323
Yang C.-T., Ma R., Axton R.A., Jackson M., Taylor A.H., Fidanza A., Marenah L., Frayne J., Mountford J.C., Forrester L.M.
Activation of KLF1 enhances the differentiation and maturation of red blood cells from human pluripotent stem cells.
Stem Cells 35:886-897(2017)

PubMed=30787325; DOI=10.1038/s41467-019-08705-0; PMCID=PMC6382809
Lopez-Yrigoyen M.B., Yang C.-T., Fidanza A., Cassetta L., Taylor A.H., McCahill A., Sellink E., von Lindern M., van den Akker E., Mountford J.C., Pollard J.W., Forrester L.M.
Genetic programming of macrophages generates an in vitro model for the human erythroid island niche.
Nat. Commun. 10:881.1-881.11(2019)

Cross-references
Encyclopedic resources Wikidata; Q111733493
Entry history
Entry creation17-Mar-2022
Last entry update10-Sep-2024
Version number5