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Cellosaurus MARC-145 PBD-1+EGFP clone 9/D6 (CVCL_B6C8)

[Text version]
Cell line name MARC-145 PBD-1+EGFP clone 9/D6
Synonyms 9/D6
Accession CVCL_B6C8
Resource Identification Initiative To cite this cell line use: MARC-145 PBD-1+EGFP clone 9/D6 (RRID:CVCL_B6C8)
Comments Group: Non-human primate cell line.
Problematic cell line: Misidentified. Grand-parent cell line (MA-104) has been shown to be from Chlorocebus pygerythrus instead of Rhesus macaque.
Characteristics: Transfected with a Neo-CMV-DEFB1-IRES-EGFP construct (PubMed=25297006).
Genetic integration: Method=Transfection; Gene=VGNC; VGNC:87240; Pig DEFB1.
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Chlorocebus pygerythrus (Vervet monkey) (Cercopithecus pygerythrus) (NCBI Taxonomy: 60710)
Hierarchy Parent: CVCL_4540 (MARC-145)
Sex of cell Sex unspecified
Age at sampling Fetus
Category Spontaneously immortalized cell line
Publications

PubMed=25297006; DOI=10.1007/s10616-014-9769-1; PMCID=PMC4754255
Huang H.-J., Peng X., Deng B., Huang C., Li J., Qian Y.-G., Gao Q.-S., Xiang M., Lu S., Chen Z.-H., Zhan C.-Y., Zhou L., Tao B.-F., Liu J., Tan B.-Z.
Fluorescent labeling for clonal selection of Marc 145 cells secreting high levels of recombinant protein PBD-1.
Cytotechnology 68:203-211(2016)

Cross-references
Encyclopedic resources Wikidata; Q112930070
Entry history
Entry creation17-Mar-2022
Last entry update19-Dec-2024
Version number7