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Cellosaurus BALB/3T3 clone A31 THO2 (CVCL_B5VI)

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Cell line name BALB/3T3 clone A31 THO2
Synonyms THO2; THO-2
Accession CVCL_B5VI
Resource Identification Initiative To cite this cell line use: BALB/3T3 clone A31 THO2 (RRID:CVCL_B5VI)
Comments Characteristics: Deficient in hypoxanthine-guanine phosphoribosyltransferase (HGPRT).
Doubling time: 30 hours (PubMed=1028169); 16 hours (Note=In 10% FBS), 20.4 hours (Note=In 1% FBS) (PubMed=701383).
Selected for resistance to: ChEBI; CHEBI:472805; Ouabain.
Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_B5VH (BALB/3T3 clone A31 NTG2)
Sex of cell Sex unspecified
Age at sampling 14-17FD
Category Transformed cell line
Publications

PubMed=1028169; DOI=10.1007/BF01538960
Jha K.K., Ozer H.L.
Expression of transformation in cell hybrids. I. Isolation and application of density-inhibited Balb/3T3 cells deficient in hypoxanthine phosphoribosyltransferase and resistant to ouabain.
Somatic Cell Genet. 2:215-223(1976)

PubMed=701383; DOI=10.1002/jcp.1040970203
Jha K.K., Cacciapuoti J., Ozer H.L.
Expression of transformation in cell hybrids. II. Nonsuppression of the transformed phenotype in hybrids between a chemically transformed and nontransformed derivatives of Balb/3T3.
J. Cell. Physiol. 97:147-152(1978)

PubMed=6933701; DOI=10.1007/BF01538640
Jha K.K., Siniscalco M., Ozer H.L.
Temperature-sensitive mutants of Balb/3T3 cells. III. Hybrids between ts2 and other mouse mutant cells affected in DNA synthesis and correction of ts2 defect by human X chromosome.
Somatic Cell Genet. 6:603-614(1980)

Cross-references
Encyclopedic resources Wikidata; Q111732947
Entry history
Entry creation17-Mar-2022
Last entry update29-Jun-2023
Version number4