ID   TK-1 [Human astrocytoma]
AC   CVCL_B325
DR   BioSample; SAMN03151978
DR   cancercelllines; CVCL_B325
DR   Cosmic; 687587
DR   Wikidata; Q54972517
RX   PubMed=9183640;
CC   Problematic cell line: Contaminated. Shown to be a U-251MG derivative. Originally thought to originate from a 22 year old female patient with a glioblastoma.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00348.
CC   Population: Caucasian.
CC   Sequence variation: Mutation; HGNC; 9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (from parent cell line).
CC   Discontinued: JCRB; NIHS0181; true.
CC   Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
ST   Source(s): JCRB
ST   Amelogenin: X,Y
ST   CSF1PO: 11,12
ST   D13S317: 10,11
ST   D16S539: 12
ST   D5S818: 11,12
ST   D7S820: 10,12
ST   TH01: 9.3
ST   TPOX: 8
ST   vWA: 16,18
DI   NCIt; C60781; Astrocytoma
DI   ORDO; Orphanet_94; Astrocytoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0021 ! U-251MG
SX   Male
AG   75Y
CA   Cancer cell line
DT   Created: 06-06-12; Last updated: 05-10-23; Version: 24
RX   PubMed=9183640;
RA   Tsuboi K., Yoshii Y., Nose T.;
RT   "Establishment of human glioblastoma cell line 'TK-1'.";
RL   Hum. Cell 9:125-128(1996).