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Cellosaurus HUES 65 (CVCL_B200)

[Text version]
Cell line name HUES 65
Synonyms HUES65; HuES65
Accession CVCL_B200
Resource Identification Initiative To cite this cell line use: HUES 65 (RRID:CVCL_B200)
Comments From: Harvard University; Boston; USA.
Registration: NIH Human Embryonic Stem Cell Registry; NIHhESC-10-0056.
Omics: Genomics; Whole exome sequencing.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
STR profile Source(s): PubMed=19200798

Markers:
AmelogeninX,Y
CSF1PO11,12
D2S133819,20
D3S135816,18,20
D5S81812,13
D7S8209,12
D8S117913,14,15
D13S3179
D16S53912,13
D19S43314
D21S1130
FGA21,25
TH018,9.3
TPOX8
vWA16,19

Run an STR similarity search on this cell line
Publications

PubMed=19200798; DOI=10.1016/j.stem.2008.12.001; PMCID=PMC3335201
Chen A.E., Egli D.M., Niakan K.K., Deng J., Akutsu H., Yamaki M., Cowan C.A., Fitz-Gerald C., Zhang K., Melton D.A., Eggan K.C.
Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines.
Cell Stem Cell 4:103-106(2009)

PubMed=21295703; DOI=10.1016/j.cell.2010.12.032; PMCID=PMC3063454
Bock C., Kiskinis E., Verstappen G., Gu H.-C., Boulting G.L., Smith Z.D., Ziller M.J., Croft G.F., Amoroso M.W., Oakley D.H., Gnirke A., Eggan K.C., Meissner A.
Reference maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.
Cell 144:439-452(2011)

PubMed=28445466; DOI=10.1038/nature22312; PMCID=PMC5427175
Merkle F.T., Ghosh S., Kamitaki N., Mitchell J., Avior Y., Mello C., Kashin S., Mekhoubad S., Ilic D., Charlton M., Saphier G., Handsaker R.E., Genovese G., Bar S., Benvenisty N., McCarroll S.A., Eggan K.C.
Human pluripotent stem cells recurrently acquire and expand dominant negative p53 mutations.
Nature 545:229-233(2017)

Cross-references
Cell line databases/resources NIHhESC; NIHhESC-10-0056
SKIP; SKIP001552
Biological sample resources ENCODE; ENCBS155QMO
Encyclopedic resources Wikidata; Q54896804
Experimental variables resources EFO; EFO_0007091
Gene expression databases GEO; GSM621599
GEO; GSM637773
Entry history
Entry creation06-Jun-2012
Last entry update10-Apr-2025
Version number23