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Cellosaurus WAe001-A-66 (CVCL_B0WC)

[Text version]
Cell line name WAe001-A-66
Synonyms H1-AAVS1-EF1a-Akaluc-KI; AkalucHES; GIBHe007-A-66
Accession CVCL_B0WC
Resource Identification Initiative To cite this cell line use: WAe001-A-66 (RRID:CVCL_B0WC)
Comments From: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences; Guangzhou; China.
Characteristics: Using CRISPR/Cas9 a construct containing the Akaluc gene under the control of a EF1a promoter was introduced in the AAVS1 safe harbor locus.
Genetic integration: Method=CRISPR/Cas9; Gene=Akaluciferase (Akaluc) (Note=A luciferase-derived fluorescent protein).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=34509917; DOI=10.1016/j.scr.2021.102532
Zhou M., Xing Q., Zhang D., Zhang C., Zhang Y.-Q., Zhang J.-Y., Shan Y.-L.
Generation of an Akaluc knock-in human embryonic stem cell reporter line using CRISPR-Cas9 technology.
Stem Cell Res. 56:102532-102532(2021)

Cross-references
Cell line databases/resources hPSCreg; WAe001-A-66
Encyclopedic resources Wikidata; Q108821503
Entry history
Entry creation23-Sep-2021
Last entry update10-Sep-2024
Version number8