Cellosaurus logo
expasy logo

Cellosaurus Expi293F Inducible GnTI- (CVCL_B0J9)

[Text version]
Cell line name Expi293F Inducible GnTI-
Accession CVCL_B0J9
Resource Identification Initiative To cite this cell line use: Expi293F Inducible GnTI- (RRID:CVCL_B0J9)
Comments Group: Serum/protein free medium cell line.
Characteristics: Lacks N-acetylglucosaminyltransferase I activity and accordingly predominantly modifies glycoproteins with the Man5GlcNAc2 N-glycan.
Characteristics: Stably expresses high levels of tetR. When used with compatible inducible vectors the gene of interest is repressed with very low levels of basal expression until induction with tetracycline. Expression can be modulated by addition of different amounts of tetracycline (Thermo Fisher).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B0J7 (Expi293F GnTI-)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Web pages https://www.thermofisher.com/order/catalog/product/A39242
Cross-references
Encyclopedic resources Wikidata; Q108820207
Entry history
Entry creation23-Sep-2021
Last entry update10-Sep-2024
Version number8