ID   ART [Human OVCAR-8 contaminated cell line]
AC   CVCL_B0CC
DR   ATCC; CRL-3468
DR   cancercelllines; CVCL_B0CC
DR   Wikidata; Q108819855
RX   PubMed=23843634;
RX   PubMed=29091757;
CC   Problematic cell line: Contaminated. Parent cell line (NCI-ADR-RES) has been shown to be a OVCAR-8 derivative.
CC   Population: Caucasian.
CC   Characteristics: Engineered to overexpress the SV40 large T antigen.
CC   Virology: Can be used to evaluate the infectious entry of polyomaviruses (BKPyV, JCPyV, SV40, MCPyV) and human papillomaviruses. Pathogenic variants of the JCPyV virus previously believed to be non-infectious can infect this cell line (ATCC=CRL-3468).
CC   Doubling time: ~34 hours (ATCC=CRL-3468).
CC   Sequence variation: Mutation; HGNC; 2514; CTNNB1; Simple; p.Gln26Arg (c.77A>G); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 3430; ERBB2; Simple; p.Gly776Val (c.2327G>T); ClinVar=VCV000163410; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Pro121His (c.362C>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; c.376-1G>A (p.Tyr126_Lys132del, c.376_396del21); ClinVar=VCV000481003; Zygosity=Homozygous; Note=Splice acceptor mutation (from parent cell line).
CC   Transfected with: UniProtKB; P03070; SV40 large T antigen.
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
ST   Source(s): ATCC
ST   Amelogenin: X
ST   CSF1PO: 11
ST   D13S317: 12
ST   D16S539: 13
ST   D5S818: 12
ST   D7S820: 20
ST   TH01: 7
ST   TPOX: 8
ST   vWA: 16,17
DI   NCIt; C105555; High grade ovarian serous adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_1629 ! OVCAR-8
SX   Female
AG   64Y
CA   Cancer cell line
DT   Created: 23-09-21; Last updated: 05-10-23; Version: 7
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RX   PubMed=23843634; DOI=10.1128/JVI.01189-13;
RA   Pastrana D.V., Ray U., Magaldi T.G., Schowalter R.M., Cuburu N.,
RA   Buck C.B.;
RT   "BK polyomavirus genotypes represent distinct serotypes with distinct
RT   entry tropism.";
RL   J. Virol. 87:10105-10113(2013).
//
RX   PubMed=29091757; DOI=10.1016/j.celrep.2017.10.027;
RA   Geoghegan E.M., Pastrana D.V., Schowalter R.M., Ray U., Gao W., Ho M.,
RA   Pauly G.T., Sigano D.M., Kaynor C., Cahir-McFarland E.,
RA   Combaluzier B., Grimm J., Buck C.B.;
RT   "Infectious entry and neutralization of pathogenic JC
RT   polyomaviruses.";
RL   Cell Rep. 21:1169-1179(2017).
//