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Cellosaurus NM2C5 GFP (CVCL_B065)

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Cell line name NM2C5 GFP
Synonyms NM2C5-GFP; NM-2C5-GFP
Accession CVCL_B065
Resource Identification Initiative To cite this cell line use: NM2C5 GFP (RRID:CVCL_B065)
Comments Problematic cell line: Contaminated. Grand-parent cell line (MDA-MB-435) has been shown to be a M14 derivative.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00564.
Population: Caucasian.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
Sequence variations
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B064 (NM2C5)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2919

Markers:
AmelogeninX
CSF1PO11
D2S133819,24
D3S135814
D5S81811,12
D7S8208,10
D8S117913
D13S31712
D16S53913
D18S5113,17
D19S43314
D21S1130
FGA21
Penta D9,11
Penta E10,12
TH016,7
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/atcc-htb-129-derivative
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=14506175
Steve Goodison, Kanji Kawai, Jun Hihara, Ping Jiang, Meng Yang, Virginia Urquidi, Robert M. Hoffman, David Tarin;
Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from nonmetastatic breast tumors as revealed by labeling with green fluorescent protein.
Clin. Cancer Res. 9:3808-3814(2003)

Cross-references
Cell line collections (Providers) ATCC; CRL-2919
Cell line databases/resources cancercelllines; CVCL_B065
Anatomy/cell type resources BTO; BTO_0006145
Encyclopedic resources Wikidata; Q54930823
Entry history
Entry creation06-Jun-2012
Last entry update14-Aug-2025
Version number31