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Cellosaurus M4A4 LM3-2 GFP (CVCL_B059)

[Text version]
Cell line name M4A4 LM3-2 GFP
Accession CVCL_B059
Resource Identification Initiative To cite this cell line use: M4A4 LM3-2 GFP (RRID:CVCL_B059)
Comments Problematic cell line: Contaminated. Ancestral cell line (MDA-MB-435) has been shown to be a M14 derivative.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00562.
Transfected with: UniProtKB; P42212; GFP.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Derived from metastatic site: Right buttock; hypodermis.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B058 (M4A4 GFP)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): ATCC

Markers:
AmelogeninX
CSF1PO11
D5S81811,12
D7S8208,10
D13S31712
D16S53913
TH016,7
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages https://www.atcc.org/en/support/technical-support/faqs/atcc-htb-129-derivative
https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Cross-references
Cell line collections ATCC; CRL-2916
Encyclopedic resources Wikidata; Q54903624
Entry history
Entry creation06-Jun-2012
Last entry update21-Mar-2023
Version number23