ID   MDA-MB-435-LCC15
AC   CVCL_B055
AS   CVCL_1U14
SY   LLC-15MB; LCC15-MB
DR   BioSample; SAMN03151830
DR   cancercelllines; CVCL_B055
DR   Wikidata; Q54904634
RX   PubMed=9637769;
RX   PubMed=10432004;
RX   PubMed=15679051;
RX   PubMed=20143388;
WW   https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
CC   Problematic cell line: Contaminated. Parent cell line (MDA-MB-435) has been shown to be a M14 derivative (PubMed=20143388). Was first thought to originate from the femoral metastasis of a breast carcinoma but was later shown to be contaminated by MDA-MB-435 (PubMed=15679051).
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00231.
CC   Population: Caucasian.
CC   Sequence variation: Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
CC   Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
DI   NCIt; C3802; Amelanotic melanoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0417 ! MDA-MB-435
SX   Male
AG   33Y
CA   Cancer cell line
DT   Created: 06-06-12; Last updated: 05-10-23; Version: 26
//
RX   PubMed=9637769; DOI=10.1006/excr.1998.4029;
RA   Sung V., Gilles C., Murray A., Clarke R., Aaron A.D., Azumi N.,
RA   Thompson E.W.;
RT   "The LCC15-MB human breast cancer cell line expresses osteopontin and
RT   exhibits an invasive and metastatic phenotype.";
RL   Exp. Cell Res. 241:273-284(1998).
//
RX   PubMed=10432004; DOI=10.1023/A:1006598422203;
RA   Thompson E.W., Sung V., Lavigne M., Baumann K.H., Azumi N., Aaron A.D.,
RA   Clarke R.;
RT   "LCC15-MB: a vimentin-positive human breast cancer cell line from a
RT   femoral bone metastasis.";
RL   Clin. Exp. Metastasis 17:193-204(1999).
//
RX   PubMed=15679051; DOI=10.1007/s10585-004-4117-z;
RA   Thompson E.W., Waltham M.C., Ramus S.J., Hutchins A.-M., Armes J.E.,
RA   Campbell I.G., Williams E.D., Thompson P.R., Rae J.M., Johnson M.D.,
RA   Clarke R.;
RT   "LCC15-MB cells are MDA-MB-435: a review of misidentified breast and
RT   prostate cell lines.";
RL   Clin. Exp. Metastasis 21:535-541(2004).
//
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A.,
RA   MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R.,
RA   Freshney R.I.;
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).
//