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Cellosaurus NRK-4xlambdaN22-3xmEGFP-M9 (CVCL_AV97)

[Text version]
Cell line name NRK-4xlambdaN22-3xmEGFP-M9
Synonyms NRK 4xlN22-3xmEGFP-M9
Accession CVCL_AV97
Resource Identification Initiative To cite this cell line use: NRK-4xlambdaN22-3xmEGFP-M9 (RRID:CVCL_AV97)
Comments Characteristics: Transfected with a construct containing four copies to the 22-amino-acid peptide ligand from phage lambda N protein (lambda-N22), spaced by a 21 amino acid linker (PPLDGAGAGAGAGAGAGGLAT) and fused to 3 monomeric meGFP and a M9 nuclear localization signal. This construct allows binding to a 15-nt RNA hairpin termed boxB that can be fused to any RNA molecule allowing specific RNA visualization in live cells (PubMed=17603490).
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03045; Escherichia phage lambda N (Note=p.Met1_Asn22 = lambdaN22).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Osborne-Mendel; RS=RS:0000629.
Hierarchy Parent: CVCL_3758 (NRK)
Category Spontaneously immortalized cell line
Publications

PubMed=17603490; DOI=10.1038/nmeth1065
Daigle N., Ellenberg J.
LambdaN-GFP: an RNA reporter system for live-cell imaging.
Nat. Methods 4:633-636(2007)

Cross-references
Cell line collections (Providers) CLS; 500672
Encyclopedic resources Wikidata; Q54931029
Entry history
Entry creation02-May-2016
Last entry update19-Dec-2024
Version number10