Cellosaurus NRK-4xlambdaN22-3xmEGFP-M9 (CVCL_AV97)
| Cell line name | NRK-4xlambdaN22-3xmEGFP-M9 |
|---|---|
| Synonyms | NRK 4xlN22-3xmEGFP-M9 |
| Accession | CVCL_AV97 |
| Resource Identification Initiative | To cite this cell line use: NRK-4xlambdaN22-3xmEGFP-M9 (RRID:CVCL_AV97) |
| Comments | Characteristics: Transfected with a construct containing four copies to the 22-amino-acid peptide ligand from phage lambda N protein (lambda-N22), spaced by a 21 amino acid linker (PPLDGAGAGAGAGAGAGGLAT) and fused to 3 monomeric meGFP and a M9 nuclear localization signal. This construct allows binding to a 15-nt RNA hairpin termed boxB that can be fused to any RNA molecule allowing specific RNA visualization in live cells (PubMed=17603490). Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P03045; Escherichia phage lambda N (Note=p.Met1_Asn22 = lambdaN22). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Kidney; UBERON=UBERON_0002113. |
| Species of origin | Rattus norvegicus (Rat)
(NCBI Taxonomy: 10116)
Breed/subspecies: Osborne-Mendel; RS=RS:0000629. |
| Hierarchy | Parent: CVCL_3758 (NRK) |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=17603490; DOI=10.1038/nmeth1065 |
| Cross-references | |
| Cell line collections (Providers) | CLS; 500672 |
| Encyclopedic resources | Wikidata; Q54931029 |
| Entry history | |
| Entry creation | 02-May-2016 |
| Last entry update | 19-Dec-2024 |
| Version number | 10 |