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Cellosaurus CHO C1s-/- MGAT1- BaL-rgp120 (CVCL_A9NZ)

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Cell line name CHO C1s-/- MGAT1- BaL-rgp120
Synonyms BaL-rgp120 C1s-/- MGAT1- CHO
Accession CVCL_A9NZ
Resource Identification Initiative To cite this cell line use: CHO C1s-/- MGAT1- BaL-rgp120 (RRID:CVCL_A9NZ)
Comments Group: Serum/protein free medium cell line.
Characteristics: Produces an unclipped gp120 from a clade B isolate (BaL-rgp120) and enriched for mannose-5 glycans. The gp120 construct contains an N-terminal purification tag from HSV-1 glycoprotein D (gD) (PubMed=31087560).
Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1.
Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3GUR0; C1s.
Transfected with: UniProtKB; Q78225; HIV-1 isolate BaL env.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_VP59 (CHO MGAT1-)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line

PubMed=31087560; DOI=10.1002/bit.27016
Li S.W., Yu B., Byrne G., Wright M., O'Rourke S.M., Mesa K.A., Berman P.W.
Identification and CRISPR/Cas9 inactivation of the C1s protease responsible for proteolysis of recombinant proteins produced in CHO cells.
Biotechnol. Bioeng. 116:2130-2145(2019)

Cell line collections (Providers) HIVReagentProgram; ARP-13573
Encyclopedic resources Wikidata; Q108820027
Entry history
Entry creation23-Sep-2021
Last entry update29-Jun-2023
Version number4