Cellosaurus CHO C1s-/- MGAT1- BaL-rgp120 (CVCL_A9NZ)
Cell line name | CHO C1s-/- MGAT1- BaL-rgp120 |
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Synonyms | BaL-rgp120 C1s-/- MGAT1- CHO |
Accession | CVCL_A9NZ |
Resource Identification Initiative | To cite this cell line use: CHO C1s-/- MGAT1- BaL-rgp120 (RRID:CVCL_A9NZ) |
Comments | Group: Serum/protein free medium cell line. Characteristics: Produces an unclipped gp120 from a clade B isolate (BaL-rgp120) and enriched for mannose-5 glycans. The gp120 construct contains an N-terminal purification tag from HSV-1 glycoprotein D (gD) (PubMed=31087560). Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1. Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3GUR0; C1s. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q78225; HIV-1 isolate BaL env. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064. |
Species of origin | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029) |
Hierarchy | Parent: CVCL_VP59 (CHO MGAT1-) |
Sex of cell | Female |
Age at sampling | Adult |
Category | Spontaneously immortalized cell line |
Publications | PubMed=31087560; DOI=10.1002/bit.27016; PMCID=PMC6675663 |
Cross-references | |
Cell line collections (Providers) | BEI_Resources; ARP-13573 |
Encyclopedic resources | Wikidata; Q108820027 |
Entry history | |
Entry creation | 23-Sep-2021 |
Last entry update | 10-Sep-2024 |
Version number | 6 |