ID   CHO C1s-/- MGAT1-
AC   CVCL_A9NX
SY   C1s-/- MGAT1- CHO; CHO.K1.MGAT1-Prot-; CHO-s-MGAT1-Protease-
DR   HIVReagentProgram; ARP-13574
DR   Wikidata; Q108820026
RX   PubMed=31087560;
CC   Group: Serum/protein free medium cell line.
CC   Characteristics: This cell line minimizes proteolysis of clade B HIV-1 envelope proteins commonly observed in the production of monomeric gp120 and gp140 as well as gp140 trimers.
CC   Characteristics: This cell line can only produce N-linked carbohydrates with early oligomannose glycans (Man5-9).
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1.
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3GUR0; C1s.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_VP59 ! CHO MGAT1-
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 23-09-21; Last updated: 30-01-24; Version: 5
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RX   PubMed=31087560; DOI=10.1002/bit.27016;
RA   Li S.W., Yu B., Byrne G., Wright M., O'Rourke S.M., Mesa K.A.,
RA   Berman P.W.;
RT   "Identification and CRISPR/Cas9 inactivation of the C1s protease
RT   responsible for proteolysis of recombinant proteins produced in CHO
RT   cells.";
RL   Biotechnol. Bioeng. 116:2130-2145(2019).
//