Cellosaurus cell line MDA-MB-231 NAT1+/- 2-12 (CVCL

Cellosaurus MDA-MB-231 NAT1+/- 2-12 (CVCL_A9BD)

Cross-references
Cell line name	MDA-MB-231 NAT1+/- 2-12
Synonyms	CRISPR 2-12
Accession	CVCL_A9BD
Resource Identification Initiative	To cite this cell line use: MDA-MB-231 NAT1+/- 2-12 (RRID:CVCL_A9BD)
Comments	Group: Triple negative breast cancer (TNBC) cell line. Population: Caucasian. Characteristics: Transfected with a scrambled NAT1 shRNA. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:7645; NAT1 (Note=1 of 2 alleles). Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations	Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line). Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Gly464Val (c.1391G>T); ClinVar=VCV000040364; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg280Lys (c.839G>A); ClinVar=VCV000376657; Zygosity=Homozygous (from parent cell line).
Disease	Breast adenocarcinoma (NCIt: C5214)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_A9BA (MDA-MB-231 NAT1 scrambled)
Sex of cell	Female
Age at sampling	51Y
Category	Cancer cell line
Publications	PubMed=32555504; DOI=10.1038/s41598-020-66863-4; PMCID=PMC7299936 Carlisle S.M., Trainor P.J., Hong K.U., Doll M.A., Hein D.W. CRISPR/Cas9 knockout of human arylamine N-acetyltransferase 1 in MDA-MB-231 breast cancer cells suggests a role in cellular metabolism. Sci. Rep. 10:9804-9804(2020)
Cell line databases/resources	cancercelllines; CVCL_A9BD
Encyclopedic resources	Wikidata; Q108820876
Entry history
Entry creation	23-Sep-2021
Last entry update	19-Dec-2024
Version number	8