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Cellosaurus MDA-MB-231 NAT1 down (CVCL_A9BB)

[Text version]
Cell line name MDA-MB-231 NAT1 down
Synonyms NAT1 shRNA Clone 3; NAT1 Knockdown
Accession CVCL_A9BB
Resource Identification Initiative To cite this cell line use: MDA-MB-231 NAT1 down (RRID:CVCL_A9BB)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Population: Caucasian.
Characteristics: Transfected with a pcDNA5/FRT derived construct with a NATb promoter driving the expression of NAT1 (NATb/NAT1*4).
Doubling time: 27.2 hours (PubMed=29315819).
Knockout cell: Method=shRNA knockdown; HGNC; HGNC:7645; NAT1.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:7645; NAT1.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Gly464Val (c.1391G>T); ClinVar=VCV000040364; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg280Lys (c.839G>A); ClinVar=VCV000376657; Zygosity=Homozygous (from parent cell line).
Disease Breast adenocarcinoma (NCIt: C5214)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0062 (MDA-MB-231)
Sex of cell Female
Age at sampling 51Y
Category Cancer cell line
Publications

PubMed=29315819; DOI=10.1002/mc.22779; PMCID=PMC5832614
Stepp M.W., Doll M.A., Carlisle S.M., States J.C., Hein D.W.
Genetic and small molecule inhibition of arylamine N-acetyltransferase 1 reduces anchorage-independent growth in human breast cancer cell line MDA-MB-231.
Mol. Carcinog. 57:549-558(2018)

PubMed=29964355; DOI=10.1002/mc.22869; PMCID=PMC6353662
Carlisle S.M., Trainor P.J., Doll M.A., Stepp M.W., Klinge C.M., Hein D.W.
Knockout of human arylamine N-acetyltransferase 1 (NAT1) in MDA-MB-231 breast cancer cells leads to increased reserve capacity, maximum mitochondrial capacity, and glycolytic reserve capacity.
Mol. Carcinog. 57:1458-1466(2018)

PubMed=32555504; DOI=10.1038/s41598-020-66863-4; PMCID=PMC7299936
Carlisle S.M., Trainor P.J., Hong K.U., Doll M.A., Hein D.W.
CRISPR/Cas9 knockout of human arylamine N-acetyltransferase 1 in MDA-MB-231 breast cancer cells suggests a role in cellular metabolism.
Sci. Rep. 10:9804-9804(2020)

Cross-references
Cell line databases/resources cancercelllines; CVCL_A9BB
Encyclopedic resources Wikidata; Q108820869
Entry history
Entry creation23-Sep-2021
Last entry update19-Dec-2024
Version number9