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Cellosaurus WISH-Mx2/EGFP (CVCL_A8RR)

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Cell line name WISH-Mx2/EGFP
Synonyms WISH-Mx2/eGFP; WISH-Mx/eGFP; L1G3
Accession CVCL_A8RR
Resource Identification Initiative To cite this cell line use: WISH-Mx2/EGFP (RRID:CVCL_A8RR)
Comments Problematic cell line: Contaminated. Parent cell line (WISH) has been shown to be a HeLa derivative.
Population: African American.
Biotechnology: Used for a reporter gene assay (RGA) of the human type 1 interferon potency (PubMed=22549076).
Characteristics: Expression of eGFP is under the control of the control of the mouse Mx2 promoter (PubMed=22549076).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1909 (WISH)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Publications

PubMed=22549076; DOI=10.1016/j.jim.2012.04.010
Maria Burgi, Claudio Prieto, Marina Etcheverrigaray, Ricardo Kratje, Marcos Oggero, Mariela Bollati-Fogolin;
WISH cell line: from the antiviral system to a novel reporter gene assay to test the potency of human IFN-alpha and IFN-beta.
J. Immunol. Methods 381:70-74(2012)

PubMed=27346232; DOI=10.1016/j.jbiotec.2016.06.021
Maria Burgi, Viktor A. Zapol'skii, Bettina Hinkelmann, Mario Koster, Dieter E. Kaufmann, Florenz Sasse, Hansjorg Hauser, Marina Etcheverrigaray, Ricardo Kratje, Mariela Bollati-Fogolin, Marcos Oggero;
Screening and characterization of molecules that modulate the biological activity of IFNs-I.
J. Biotechnol. 233:6-16(2016)

PubMed=31699391; DOI=10.1016/j.bioorg.2019.103372
Maria Burgi, Paola Hernandez, Mauricio Cabrera, Hugo Cerecetto, Mercedes Gonzalez, Ricardo Kratje, Alejandro Raimondi, Marcos Oggero, Mariela Bollati-Fogolin;
Identification and characterization of human interferon alpha inhibitors through a WISH cell line-based reporter gene assay.
Bioorg. Chem. 94:103372.1-103372.10(2020)

Cross-references
Encyclopedic resources Wikidata; Q108821517
Entry history
Entry creation23-Sep-2021
Last entry update14-Aug-2025
Version number8